Purification of a membrane-bound sorbitol dehydrogenase fromGluconobacter suboxydans
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چکیده
منابع مشابه
Sorbitol dehydrogenases in Acetobacter suboxydans.
It has previously been shown that cell-free extracts of Acetobacter subozydans possess two pathways for the oxidation of sorbitol (1). In the presence of DPN,l fructose is produced; with TPN, sorbose is formed. The primary purpose of this paper is to describe the separation of these two dehydrogenases from each other. The DPN enzyme free from TPN activity has been concentrated about lBfold, whe...
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NAD-dependent D-sorbitol dehydrogenase (EC 1.1.1.14) was crystallized from the cytosolic fraction of Gluconobacter suboxydans IFO 3257. This is the first example of the enzyme crystallized from acetic acid bacteria. The enzyme catalyzed oxidoreduction between D-sorbitol and D-fructose in the presence of NAD or NADH. The crystalline enzyme showed a single sedimentation peak in analytical ultrace...
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with n-heptyl-fi-D-thioglucoside, and then purified by DEAE-Sepharose and hydroxylapatite columns containing Triton X-100. The puTified enzyme was electrophoretically homogeneous. The enzyme had an apparent molecular mass of 400kDa, and was composed of three subunits whose molecular masses were 74, 70, and 62 kDa. The purified enzyme was much more labile than the membrane-bound enzyme (membrane...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1995
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1995.tb07333.x